Phenotypic expression of HA-NA combinations in human-avian influenza A virus reassortants
Identifieur interne : 001C55 ( Main/Exploration ); précédent : 001C54; suivant : 001C56Phenotypic expression of HA-NA combinations in human-avian influenza A virus reassortants
Auteurs : I. A. Rudneva [Russie] ; E. I. Sklyanskaya [Russie] ; O. S. Barulina [Russie] ; S. S. Yamnikova [Russie] ; V. P. Kovaleva [Russie] ; I. V. Tsvetkova [Russie] ; N. V. Kaverin [Russie]Source :
- Archives of Virology [ 0304-8608 ] ; 1996-06-01.
English descriptors
- Teeft :
- Actual virion yields, Aggregation, Allantoic, Allantoic fluid, Chick embryos, Embryonated chicken eggs, Enzymatic activity, Hemagglutinin, Influenza, Influenza virus, Initial reassortants, Monoclonal antibodies, Natural conditions, Neuraminidase, Other genes, Parent virus, Parent viruses, Passage variants, Passaged, Passaged variant, Passaged variants, Previous communication, Reassortant, Reassortants, Rudneva, Rwb1, Serial passages, Subtypes, Such reassortants, Sucrose, Sucrose cushion, Titer, Variant, Virion, Virion aggregates, Virion aggregation, Virus, Virus reassortants, Virus yields.
Abstract
Summary: Human-avian and human-mammalian influenza A virus reassortant clones with the neuraminidase (NA) gene of the A/USSR/90/77 (H1N1) strain and hemagglutinin (HA) genes of H3, H4 and H13 subtypes had been shown in an earlier publication to produce low HA yields in the embryonated chicken eggs. The low HA titers had been shown to be due, at least in part, to the formation of virion clusters at 4°C; the clustering was removed by the treatment with bacterial neuraminidase [Rudneva et al., Arch. Virol (1993) 133: 437–450]. By serial passages of the reassortants in chick embryos non-aggregating variants were selected: the variants produced HA titers of the same order as A/USSR/90/77 parent virus. The assessment of the virus yields by the analysis of the partially purified virus preparations from fixed volumes of the allantoic fluid revealed that actual virion yields of the initial reassortants were lower than the yields of their passaged variants or of the parent viruses. The passaged variant of a reassortant possessing the HA gene of A/Duck/Ukraine/1/63 (H3N2) virus differed from the original (non-passaged) reassortant and from the parent A/Duck/Ukraine/1/63 virus in the reaction with a panel of monoclonal antibodies against H3 hemagglutinin. The data suggest that some HA-NA combinations may lead to an incomplete functional match between HA and NA and to the formation of low-yield reassortants, thus representing a possible limiting factor in the emergence of new HA-NA combinations in natural conditions.
Url:
DOI: 10.1007/BF01718612
Affiliations:
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The low HA titers had been shown to be due, at least in part, to the formation of virion clusters at 4°C; the clustering was removed by the treatment with bacterial neuraminidase [Rudneva et al., Arch. Virol (1993) 133: 437–450]. By serial passages of the reassortants in chick embryos non-aggregating variants were selected: the variants produced HA titers of the same order as A/USSR/90/77 parent virus. The assessment of the virus yields by the analysis of the partially purified virus preparations from fixed volumes of the allantoic fluid revealed that actual virion yields of the initial reassortants were lower than the yields of their passaged variants or of the parent viruses. The passaged variant of a reassortant possessing the HA gene of A/Duck/Ukraine/1/63 (H3N2) virus differed from the original (non-passaged) reassortant and from the parent A/Duck/Ukraine/1/63 virus in the reaction with a panel of monoclonal antibodies against H3 hemagglutinin. The data suggest that some HA-NA combinations may lead to an incomplete functional match between HA and NA and to the formation of low-yield reassortants, thus representing a possible limiting factor in the emergence of new HA-NA combinations in natural conditions.</div></front></TEI><affiliations><list><country><li>Russie</li></country><region><li>District fédéral central</li></region><settlement><li>Moscou</li></settlement></list><tree><country name="Russie"><region name="District fédéral central"><name sortKey="Rudneva, I A" sort="Rudneva, I A" uniqKey="Rudneva I" first="I. A." last="Rudneva">I. A. Rudneva</name></region><name sortKey="Barulina, O S" sort="Barulina, O S" uniqKey="Barulina O" first="O. S." last="Barulina">O. S. Barulina</name><name sortKey="Kaverin, N V" sort="Kaverin, N V" uniqKey="Kaverin N" first="N. V." last="Kaverin">N. V. Kaverin</name><name sortKey="Kovaleva, V P" sort="Kovaleva, V P" uniqKey="Kovaleva V" first="V. P." last="Kovaleva">V. P. Kovaleva</name><name sortKey="Sklyanskaya, E I" sort="Sklyanskaya, E I" uniqKey="Sklyanskaya E" first="E. I." last="Sklyanskaya">E. I. Sklyanskaya</name><name sortKey="Tsvetkova, I V" sort="Tsvetkova, I V" uniqKey="Tsvetkova I" first="I. V." last="Tsvetkova">I. V. Tsvetkova</name><name sortKey="Yamnikova, S S" sort="Yamnikova, S S" uniqKey="Yamnikova S" first="S. S." last="Yamnikova">S. S. Yamnikova</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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